Anti-Flightless I antibody [EPR4201] (ab108594)
![Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-389431-anti-flightless-i-antibody-epr4201-western-blot-human-knockout-lysate.jpg "Anti-Flightless I antibody [EPR4201] (ab108594)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4201] to Flightless I
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Flightless I antibody [EPR4201]
See all Flightless I primary antibodies -
Description
Rabbit monoclonal [EPR4201] to Flightless I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A549 and A431 cell lysates. IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4201 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FLII knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 145 kDa
Observed band size: 145 kDaLanes 1-3: Merged signal (red and green). Green - ab108594 observed at 145 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108594 Anti-Flightless I antibody [EPR4201] was shown to specifically react with Flightless I in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265890 (knockout cell lysate ab257954) was used. Wild-type and Flightless I knockout samples were subjected to SDS-PAGE. ab108594 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-121283-anti-flightless-i-antibody-epr4201-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-121282-anti-flightless-i-antibody-epr4201-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-121284-anti-flightless-i-antibody-epr4201-western-blot.gif)
Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa
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![Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-5-benefits-of-recombinant-antibodies.png)
Anti-Flightless I antibody [EPR4201] (ab108594)
