Anti-Flightless I antibody [EPR4202(2)] (ab109015)
![Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.com/ps/products/109/ab109015/Images/ab109015-121587-anti-flightless-i-antibody-epr42022-western-blot.jpg "Anti-Flightless I antibody [EPR4202(2)] (ab109015)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4202(2)] to Flightless I
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Flightless I antibody [EPR4202(2)]
See all Flightless I primary antibodies -
Description
Rabbit monoclonal [EPR4202(2)] to Flightless I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human heart, HeLa, A431, and A549 lysates; Human muscle tissue; HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4202(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Flightless I antibody [EPR4202(2)] (ab109015)All lanes : Anti-Flightless I antibody [EPR4202(2)] (ab109015) at 1/1000 dilution
Lane 1 : Humam heart lysate
Lane 2 : HeLa lysate
Lane 3 : A431 lysate
Lane 4 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.com/ps/products/109/ab109015/Images/ab109015-121586-anti-flightless-i-antibody-epr42022-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (ab109015)Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.com/ps/products/109/ab109015/Images/ab109015-121585-anti-flightless-i-antibody-epr42022-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (ab109015)Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution. -
![Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.com/ps/products/109/ab109015/Images/ab109015-4-benefits-of-recombinant-antibodies.png)
Anti-Flightless I antibody [EPR4202(2)] (ab109015)
