Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11210] to CAPZA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free
    See all CAPZA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11210] to CAPZA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CAPZA1. The exact sequence is proprietary.
    Database link: P52907

  • General notes

    Ab249376 is the carrier-free version of ab166892. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Western blot - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    All lanes : Anti-CAPZA1 antibody [EPR11210] (ab166892) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa



    This data was developed using ab166892, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)

    This data was developed using ab166892, the same antibody clone in a different buffer formulation.

    ab166892 showing +ve staining in Human thyroid gland carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)

    This data was developed using ab166892, the same antibody clone in a different buffer formulation.

    ab166892 showing +ve staining in Human urinary bladder carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)

    This data was developed using ab166892, the same antibody clone in a different buffer formulation.

    ab166892 showing +ve staining in Human colonic adenocarcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    This data was developed using ab166892, the same antibody clone in a different buffer formulation.ab166892 showing +ve staining in Human normal brain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    This data was developed using ab166892, the same antibody clone in a different buffer formulation.ab166892, at 1/1000 dilution, staining CAPZA1 in HepG2 cell lysate immunoprecipitated using ab166892 at 1/10.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    This data was developed using ab166892, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling CAPZA1 with ab166892 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    This data was developed using ab166892, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab166892 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Flow Cytometry - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    This data was developed using ab166892, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells using ab166892 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).
  • Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)
    Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (ab249376)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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