Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10739] to Activin Receptor Type IIB/ACVR2B
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739]
    See all Activin Receptor Type IIB/ACVR2B primary antibodies

  • Description

    Rabbit monoclonal [EPR10739] to Activin Receptor Type IIB/ACVR2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Activin Receptor Type IIB/ACVR2B aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13705

  • Positive control

    • WB: K562, NIH:OVCAR-3, 293T and Jurkat whole cell lysate (ab7899), mouse and rat heart tissue lysates. IP: HEK-283T whole cell lysate.

  • General notes

     This product was previously labelled as Activin Receptor Type IIB

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    Immunoprecipitation - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)

    Activin Receptor Type IIB/ACVR2B was immunoprecipitated from 0.35 mg HEK-293T ( Human embryonic kidney epithelial cell) whole cell lysate with ab180185 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab180185 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HEK-293T whole cell lysate 10 µg (Input).
    Lane 2: ab180185 IP in HEK-283T whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab180185 in HEK-293T whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185) at 1/5000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 58 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185) at 1/5000 dilution (purified)

    Lane 1 : K562 whole cell lysate
    Lane 2 : NIH:OVCAR-3 whole cell lysate
    Lane 3 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 58 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : NIH:OVCAR-3 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 58 kDa

  • Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)
    Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185)

© 2021 Astana Biomed Group LLP. All rights reserved.