Anti-CXCR4 (phospho S324 + S325) antibody [4 HCLC] (ab277778)
![Anti-CXCR4 (phospho S324 + S325) antibody [4 HCLC] (ab277778)](https://www.abcam.com/ps/products/277/ab277778/Images/ab277778-2-antiPhosphoCXCR4S324S325recombinantpolyclonalantibody4hclcWB.jpg "Anti-CXCR4 (phospho S324 + S325) antibody [4 HCLC] (ab277778)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [4 HCLC] to CXCR4 (phospho S324 + S325)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-CXCR4 (phospho S324 + S325) antibody [4 HCLC]
See all CXCR4 primary antibodies -
Description
Rabbit recombinant multiclonal [4 HCLC] to CXCR4 (phospho S324 + S325) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CXCR4 aa 300-400 (phospho S324 + S325).
Database link: P61073 -
Positive control
- WB: HEK-293, A549, MCF-7, LNCaP, HeLa extract. ICC: Jurkat cells.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
4 HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CXCR4 antibody (ab277778)All lanes : Anti-CXCR4 (phospho S324 + S325) antibody [4 HCLC] (ab277778) at 2.5 µg/ml
Lane 1 : HEK-293 extracts
Lane 2 : A549 extracts
Lane 3 : MCF-7 extract
Lane 4 : LNCaP extract
Lane 5 : HeLa extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal™, 0.25 µg/mL at 1/4000 dilution
Predicted band size: 39 kDa
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Immunocytochemistry - Anti-CXCR4 antibody (ab277778)For immunofluorescence analysis Jurkat cells were fixed and permeabilized for detection of endogenous CXCR4 [pS324/325] using Anti- CXCR4 [pS324/325] Recombinant Rabbit Mutliclonal Antibody (ab277778, 5 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI, and Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of CXCR4 [pS324/325] (green) on the membrane can be clearly observed in cells treated with PMA (40 ng/ML, 30 min) as compared to untreated cells. Antibody specificity was demonstrated by competition with the CXCR4 [pS324/325] phospho peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.
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![Anti-CXCR4 antibody [4 HCLC] (ab277778)](https://www.abcam.com/ps/products/277/ab277778/Images/ab277778-3-benefits-of-recombinant-antibodies.jpg)
Anti-CXCR4 antibody [4 HCLC] (ab277778)