HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)
![HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)](https://www.abcam.com/ps/products/190/ab190564/Images/ab190564-349843-ID1297ab190564AP46282754mDSimage.jpg "HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2241] to Cyclin D1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cyclin D1 antibody [EPR2241]
See all Cyclin D1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR2241] to Cyclin D1 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, RAW264.7 and PC12 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 2.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2241 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)All lanes : HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
Lane 1 : MCF7 Whole Cell Lysate
Lane 2 : RAW 264.7 Whole Cell Lysate
Lane 3 : PC12 Whole cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk blocking buffer before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)](https://www.abcam.com/ps/products/190/ab190564/Images/ab190564-242525-anti-cyclin-d1-antibody-epr2241-hrp-western-blot.jpg)
Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)All lanes : HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)](https://www.abcam.com/ps/products/190/ab190564/Images/ab190564-242526-anti-cyclin-d1-antibody-epr2241-hrp-western-blot.jpg)
Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)](https://www.abcam.com/ps/products/190/ab190564/Images/ab190564-4-benefits-of-recombinant-antibodies.png)
HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)
