Anti-Cyclin D1 (phospho T286) antibody (ab62151)
Key features and details
- Rabbit polyclonal to Cyclin D1 (phospho T286)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin D1 (phospho T286) antibody
See all Cyclin D1 primary antibodies -
Description
Rabbit polyclonal to Cyclin D1 (phospho T286) -
Host species
Rabbit -
Specificity
Detects endogenous levels of Cyclin D1 only when phosphorylated at threonine 286 -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human Cyclin D1 around the phosphorylation site of threonine 286 (A-C-TP-P-T).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin D1 (phospho T286) antibody (ab62151)All lanes : Anti-Cyclin D1 (phospho T286) antibody (ab62151) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins) with immunizing phosphopeptide
Predicted band size: 33 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
