Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cancer Cell cycle Cyclins Cyclin D family

Anti-Cyclin D1 antibody (ab226977)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Cyclin D1 antibody (ab226977)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cyclin D1
  • Suitable for: WB, IP, ICC/IF, Sandwich ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

You may also be interested in

Product image
Human Cyclin D1 Antibody Pair - BSA and Azide free (ab241853)
Product image
Human CCND1 (Cyclin D1) knockout HeLa cell pellet (ab279244)
Product image
Anti-Cyclin D1 antibody [EP272Y] - BSA and Azide free (ab227561)
Product image
Anti-Cyclin D1 antibody [SP4] - BSA and Azide free (ab239794)

Overview

  • Product name

    Anti-Cyclin D1 antibody
    See all Cyclin D1 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin D1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse, Rat, Cow, Dog, Rhesus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Cyclin D1.
    Database link: P24385

  • Positive control

    • WB: HeLa, MCF7 and MDA-MB-231 whole cell extracts. IP: MCF7 whole cell extract. ICC/IF: SK-N-SH cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin D Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin D Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin D family

Images

  • Western blot - Anti-Cyclin D1 antibody (ab226977)
    Western blot - Anti-Cyclin D1 antibody (ab226977)
    All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Cyclin D1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    12% SDS-PAGE gel.

  • Western blot - Anti-Cyclin D1 antibody (ab226977)
    Western blot - Anti-Cyclin D1 antibody (ab226977)
    All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 34 kDa



    12% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody (ab226977)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody (ab226977)

    4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling Cyclin D1 (green) using ab226977 at 1/500 dilution in ICC/IF.

    Counterstain: Cytoskeleton marker beta Tubulin 3/ Tuj1 antibody.

  • Immunoprecipitation - Anti-Cyclin D1 antibody (ab226977)
    Immunoprecipitation - Anti-Cyclin D1 antibody (ab226977)

    Cyclin D1 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 µg ab226977. Western blot was performed from the immunoprecipitate using ab226977. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in MCF7 whole cell extract.

    Lane 2: ab226977 IP in MCF7 whole cell extract.

  • Sandwich ELISA - Anti-Cyclin D1 antibody (ab226977)
    Sandwich ELISA - Anti-Cyclin D1 antibody (ab226977)

    Sandwich ELISA of recombinant full-length Cyclin D1 protein using ab226977 at 5μg/mL.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Cyclin D1 antibody (ab226977)

  •  
  • Product image

    Anti-Cyclin D1 antibody [EP272Y] (ab40754)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Alexa Fluor® 488 Anti-Cyclin D1 antibody [EPR2241] (ab190194)

    Applications: ICC/IF

  •  
  • Product image

    Anti-Cyclin D1 antibody [EPR2241] - C-terminal (ab134175)

    Applications: ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)

    Applications: WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-Cyclin D1 antibody [EPR2241] (ab190563)

    Applications: ICC/IF

  •  
  • Product image

    Anti-Cyclin D1 antibody [EPR2241] - BSA and Azide free (ab156448)

    Applications: ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-Cyclin D1 antibody [SP4] (ab16663)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Caldesmon/CDM antibody [E89] (ab32330)

  •  
  • Product image

    Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

  •  
  • Product image

    Anti-F4/80 antibody [BM8] (ab16911)

  •  
  • Product image

    Anti-PPP1CB antibody [EP1804Y] (ab53315)

  •  
  • Product image

    Anti-Pax2 antibody [EPR8586] (ab150391)

  •  
  • Product image

    Anti-Von Willebrand Factor antibody [EPR12010] (ab179451)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.