Anti-Cyclin D1 antibody (ab226977)
Key features and details
- Rabbit polyclonal to Cyclin D1
- Suitable for: WB, IP, ICC/IF, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Cyclin D1 antibody
See all Cyclin D1 primary antibodies -
Description
Rabbit polyclonal to Cyclin D1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Dog, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human Cyclin D1.
Database link: P24385 -
Positive control
- WB: HeLa, MCF7 and MDA-MB-231 whole cell extracts. IP: MCF7 whole cell extract. ICC/IF: SK-N-SH cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Cyclin D1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 34 kDa12% SDS-PAGE gel.
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All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 34 kDa12% SDS-PAGE
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4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling Cyclin D1 (green) using ab226977 at 1/500 dilution in ICC/IF.
Counterstain: Cytoskeleton marker beta Tubulin 3/ Tuj1 antibody.
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Cyclin D1 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 µg ab226977. Western blot was performed from the immunoprecipitate using ab226977. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab226977 IP in MCF7 whole cell extract.
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Sandwich ELISA of recombinant full-length Cyclin D1 protein using ab226977 at 5μg/mL.