Anti-Cyclin E1 antibody [5F8C5] (ab211342)
![Anti-Cyclin E1 antibody [5F8C5] (ab211342)](https://www.abcam.com/ps/products/211/ab211342/Images/ab211342-259241-anti-cyclin-e1-antibody-5f8c5-flow-cytometry.jpg "Anti-Cyclin E1 antibody [5F8C5] (ab211342)")
Key features and details
- Mouse monoclonal [5F8C5] to Cyclin E1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin E1 antibody [5F8C5]
See all Cyclin E1 primary antibodies -
Description
Mouse monoclonal [5F8C5] to Cyclin E1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Cyclin E1 aa 300 to the C-terminus.
Database link: P24864 -
Positive control
- Cyclin E1 (AA: 307-410) recombinant protein; Cyclin E1 (AA: 307-410)-hIgGFc transfected HEK293 cell lysate; HeLa, K562, NIH3T3, C6, MCF7, Jurkat and A431 cell lysates; K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5F8C5 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Cyclin E1 antibody [5F8C5] (ab211342)
Flow Cytometrical analysis of K562 cells labeling Cyclin E1 with ab211342 at 1/200 (green) compared to a negative control (red).
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![Western blot - Anti-Cyclin E1 antibody [5F8C5] (ab211342)](https://www.abcam.com/ps/products/211/ab211342/Images/ab211342-259239-anti-cyclin-e1-antibody-5f8c5-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [5F8C5] (ab211342)All lanes : Anti-Cyclin E1 antibody [5F8C5] (ab211342) at 1/500 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : NIH3T3 cell lysates
Lane 4 : C6 cell lysates
Lane 5 : MCF7 cell lysates
Lane 6 : Jurkat cell lysates
Lane 7 : A431 cell lysates
Predicted band size: 47 kDa
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![Western blot - Anti-Cyclin E1 antibody [5F8C5] (ab211342)](https://www.abcam.com/ps/products/211/ab211342/Images/ab211342-259238-anti-cyclin-e1-antibody-5f8c5-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [5F8C5] (ab211342)All lanes : Anti-Cyclin E1 antibody [5F8C5] (ab211342) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Cyclin E1 (AA: 307-410)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 47 kDa
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![Western blot - Anti-Cyclin E1 antibody [5F8C5] (ab211342)](https://www.abcam.com/ps/products/211/ab211342/Images/ab211342-259237-anti-cyclin-e1-antibody-5f8c5-western-blot.jpg)
Western blot - Anti-Cyclin E1 antibody [5F8C5] (ab211342)Anti-Cyclin E1 antibody [5F8C5] (ab211342) at 1/500 dilution + human Cyclin E1 (AA: 307-410) recombinant protein
Predicted band size: 47 kDa
Observed band size: 37.5 kDa why is the actual band size different from the predicted?
