Anti-Cyclin H/p34 (phospho T315) antibody (ab73208)
Key features and details
- Rabbit polyclonal to Cyclin H/p34 (phospho T315)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin H/p34 (phospho T315) antibody
See all Cyclin H/p34 primary antibodies -
Description
Rabbit polyclonal to Cyclin H/p34 (phospho T315) -
Host species
Rabbit -
Specificity
ab73208 detects endogenous levels of Cyclin H/p34 only when phosphorylated at threonine 315.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Cyclin H/p34.
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Positive control
- HeLa cell extract.
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General notes
This product was previously labelled as Cyclin H
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab73208 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin H/p34 (phospho T315) antibody (ab73208)All lanes : Anti-Cyclin H/p34 (phospho T315) antibody (ab73208) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
