Anti-Cyclin B1 antibody (ab215436)
Key features and details
- Rabbit polyclonal to Cyclin B1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin B1 antibody
See all Cyclin B1 primary antibodies -
Description
Rabbit polyclonal to Cyclin B1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Mouse Cyclin B1 aa 350-450 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P24860 -
Positive control
- Human colon carcinoma tissue; HeLa cells; HeLa, U937, U251 and K562 cell lysates; HEK293 and MCF-7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + HeLa cell lysates
Predicted band size: 48 kDa
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Cyclin B1 with ab215436 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining
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Flow cytometric analysis of HeLa cells labeling Cyclin B1 with ab215436 at 1/100 dilution for 60 minutes at room temperature, followed by incubation with a Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 conjugated secondary antibody.
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Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + U937 cell lysates
Predicted band size: 48 kDa
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Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + U251 cell lysates
Predicted band size: 48 kDa
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Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + K562 cell lysates
Predicted band size: 48 kDa