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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Cell cycle Cyclins

Anti-Cyclin B1 antibody (ab215436)

Anti-Cyclin B1 antibody (ab215436)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cyclin B1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cyclin B1 antibody
    See all Cyclin B1 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse Cyclin B1 aa 350-450 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P24860

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human colon carcinoma tissue; HeLa cells; HeLa, U937, U251 and K562 cell lysates; HEK293 and MCF-7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin B Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin B Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin B family

Images

  • Western blot - Anti-Cyclin B1 antibody (ab215436)
    Western blot - Anti-Cyclin B1 antibody (ab215436)
    Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + HeLa cell lysates

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody (ab215436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody (ab215436)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Cyclin B1 with ab215436 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining

  • Flow Cytometry - Anti-Cyclin B1 antibody (ab215436)
    Flow Cytometry - Anti-Cyclin B1 antibody (ab215436)

    Flow cytometric analysis of HeLa cells labeling Cyclin B1 with ab215436 at 1/100 dilution for 60 minutes at room temperature, followed by incubation with a Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 conjugated secondary antibody.

  • Western blot - Anti-Cyclin B1 antibody (ab215436)
    Western blot - Anti-Cyclin B1 antibody (ab215436)
    Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + U937 cell lysates

    Predicted band size: 48 kDa

  • Western blot - Anti-Cyclin B1 antibody (ab215436)
    Western blot - Anti-Cyclin B1 antibody (ab215436)
    Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + U251 cell lysates

    Predicted band size: 48 kDa

  • Western blot - Anti-Cyclin B1 antibody (ab215436)
    Western blot - Anti-Cyclin B1 antibody (ab215436)
    Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution + K562 cell lysates

    Predicted band size: 48 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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