Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 16, 2021

Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22328-235] to Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280)
Suitable for: WB, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235]
Description

Rabbit monoclonal [EPR22328-235] to Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HT-1080 treated with 10 µM MG-132 for 4 hours, whole cell lysate;PC-12 treated with 10 µM MG-132 for 4 hours, whole cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22328-235
Isotype

IgG
Research areas

Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

All lanes : Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818) at 1/1000 dilution

Lane 1 : Untreated NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : NIH/3T3 treated with 50 uM MG-132 for 90 minutes, whole cell lysate
Lane 3 : Untreated PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : PC-12 treated with 10 uM MG-132 for 4 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody reacts with unidentifiable proteins on the top of the membrane. Phosphorylation of cyclin D1 at T286 leads to its degradation (PMID: 22069692).

Exposure time: 3 minutes
Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Lane 1 : Untreated HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
Lane 2 : HT-1080 treated with 10 µM MG-132 for 4 hours, whole cell lysate
Lane 3 : HT-1080 treated with 10 µM MG-132 for 4 hours, whole cell lysate (Phosphatase treated membrane)

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody reacts with unidentifiable proteins on the top of the membrane.

WB membrane in lane 3 was treated with alkaline phosphatase for 1h at 37 degree.

Phosphorylation of cyclin D1 at T286 leads to its degradation (PMID: 22069692).

Exposure time: 3 minutes
Dot Blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

Dot blot analysis using ab230818 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100,000 dilution.

Lane 1: CCND1 non-phospho peptide (aa279-294)

Lane 2: CCND1 (phospho T286) peptide (aa284-294)

Lane 3: CCND2 (phospho T280) peptide (aa278-288
Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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