Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22328-235] to Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235]

  • Description

    Rabbit monoclonal [EPR22328-235] to Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-1080 treated with 10 µM MG-132 for 4 hours, whole cell lysate;PC-12 treated with 10 µM MG-132 for 4 hours, whole cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)
    Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)
    All lanes : Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : NIH/3T3 treated with 50 uM MG-132 for 90 minutes, whole cell lysate
    Lane 3 : Untreated PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 4 : PC-12 treated with 10 uM MG-132 for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody reacts with unidentifiable proteins on the top of the membrane.  Phosphorylation of cyclin D1 at T286 leads to its degradation (PMID: 22069692). 

    Exposure time: 3 minutes

  • Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)
    Western blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Lane 1 : Untreated HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
    Lane 2 : HT-1080 treated with 10 µM MG-132 for 4 hours, whole cell lysate
    Lane 3 : HT-1080 treated with 10 µM MG-132 for 4 hours, whole cell lysate (Phosphatase treated membrane)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody reacts with unidentifiable proteins on the top of the membrane.

    WB membrane in lane 3 was treated with alkaline phosphatase for 1h at 37 degree.

    Phosphorylation of cyclin D1 at T286 leads to its degradation (PMID: 22069692).

    Exposure time: 3 minutes

  • Dot Blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)
    Dot Blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

    Dot blot analysis using ab230818 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100,000 dilution.

    Lane 1: CCND1 non-phospho peptide (aa279-294)  

    Lane 2: CCND1 (phospho T286) peptide (aa284-294)

    Lane 3: CCND2 (phospho T280) peptide (aa278-288

  • Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)
    Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] (ab230818)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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