Recombinant human CDK1 + Cyclin B1 protein (Active) (ab271456)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant human CDK1 + Cyclin B1 protein (Active) -
Biological activity
Specific activity ≥200 pmol/min/μg.
Assay was done in a kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 µM ATP at 30°C for 45 min.
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Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MALRVTRNSK INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN KVSEQLQAKM PMKKEAKPSA TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE PEPEPEPEPV KEEKLSPEPI LVDTASPSPM ETSGCAPAEE DLCQAFSDVI LAVNDVDAED GADPNLCSEY VKDIYAYLRQ LEEEQAVRPK YLLGREVTGN MRAILIDWLV QVQMKFRLLQ ETMYMTVSII DRFMQNNCVP KKMLQLVGVT AMFIASKYEE MYPPEIGDFA FVTDNTYTKH QIRQMEMKIL RALNFGLGRP LPLHFLRRAS KIGEVDVEQH TLAKYLMELT MLDYDMVHFP PSQIAAGAFC LALKILDNGE WTPTLQHYLS YTEESLLPVM QHLAKNVVMV NQGLTKHMTV KNKYATSKHA KISTLPQLNS ALVQDLAKAV AKV -
Predicted molecular weight
75 kDa including tags -
Amino acids
1 to 433 -
Tags
GST tag N-Terminus -
Additional sequence information
Genbank: NM_031966
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Amino Acid Sequence 2
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Species
Human -
Sequence
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKM -
Predicted molecular weight
60 kDa including tags -
Amino acids
1 to 297 -
Tags
GST tag N-Terminus -
Additional sequence information
Genbank: NM_001786
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Preparation and Storage
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Cellular localization
CDK1: Nucleus. Cyclin B1: Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
Images
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Functional Studies - Recombinant Human CDK1 + Cyclin B1 protein (Tagged) (ab271456)
Functional analysis of ab271456.
Specific activity ≥200 pmol/min/μg.
Assay was done in a kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 µM ATP at 30°C for 45 min.
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SDS-PAGE - Recombinant Human CDK1 + Cyclin B1 protein (Tagged) (ab271456)SDS-PAGE analysis of ab271456.