Anti-Cyclin E1 antibody - BSA and Azide free (Detector) (ab244991)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to Cyclin E1 - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Cyclin E1 antibody - BSA and Azide free (Detector)
See all Cyclin E1 primary antibodies -
Description
Rabbit monoclonal to Cyclin E1 - BSA and Azide free (Detector) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
General notes
ab244991 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Cyclin E1. The recommended pair for sandwich ELISA is:
Capture: ab244724, Human Cyclin E1 Capture Antibody (unconjugated)
Detector: ab244991, Human Cyclin E1 Detector Antibody (unconjugated)
The reference range value is 3.91 - 250 ng/ml.The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cyclin E1 antibody - BSA and Azide free (Detector) (ab244991) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate
Lane 3 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate
Lane 4 : HAP1 (Human chronic myelogenous leukemia) cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 minuteBlocking Buffer and concentration: 5% NFDM/TBST
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