Rat IL-7 Antibody Pair - BSA and Azide free (ab253494)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Rat
Overview
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Product name
Rat IL-7 Antibody Pair - BSA and Azide free
See all IL7 kits -
Assay type
ELISA set -
Range
5.469 pg/ml - 350 pg/ml -
Species reactivity
Reacts with: Rat -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Rat IL-7. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277721), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Rat IL-7 Capture Antibody (unconjugated) 1 x 100µg Rat IL-7 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Relevance
Interleukin 7 (IL-7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. -
Cellular localization
Secreted -
Alternative names
- IL 7
- IL7
- Interleukin 7
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Database links
- Entrez Gene: 25647 Rat
- SwissProt: P56478 Rat
Images
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Example of Rat IL-7 standard curve.
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277721), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253494)To learn more about the advantages of recombinant antibodies see here.
