Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22816-65] to IL-2 Receptor alpha
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [EPR22816-65]
    See all IL-2 Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR22816-65] to IL-2 Receptor alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse IL-2 Receptor alpha recombinant protein, Mouse thymus and WEHI-231 lysates. Flow Cyt: Mouse splenocytes cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
    Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
    Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution + His-tagged mouse IL-2 Receptor alpha recombinant protein (aa22-236) 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This recombinant protein with LIF-tag (LIF tag MW is ~40KD) expressed from a mammalian - HEK-293 expression system.

  • Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
    Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

    Flow cytometric analysis of Mouse splenocytes cells labelling IL-2 Receptor alpha with ab264557 at 1/500 (Right) as compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab264557 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

  • Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
    Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
    All lanes : Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution

    Lane 1 : Mouse thymus lysate
    Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 3 minutes; Lane 2: 81 seconds.

  • Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
    Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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