Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

Order now and get it on Tuesday March 16, 2021

Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22816-65] to IL-2 Receptor alpha
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-IL-2 Receptor alpha antibody [EPR22816-65]
See all IL-2 Receptor alpha primary antibodies
Description

Rabbit monoclonal [EPR22816-65] to IL-2 Receptor alpha
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse IL-2 Receptor alpha recombinant protein, Mouse thymus and WEHI-231 lysates. Flow Cyt: Mouse splenocytes cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Mouse
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22816-65
Isotype

IgG
Research areas

Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution + His-tagged mouse IL-2 Receptor alpha recombinant protein (aa22-236) 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 31 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 5 seconds.

This recombinant protein with LIF-tag (LIF tag MW is ~40KD) expressed from a mammalian - HEK-293 expression system.
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

Flow cytometric analysis of Mouse splenocytes cells labelling IL-2 Receptor alpha with ab264557 at 1/500 (Right) as compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab264557 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor^® 647.

Gated on viable cells.
Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

All lanes : Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution

Lane 1 : Mouse thymus lysate
Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 31 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 3 minutes; Lane 2: 81 seconds.
Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com