Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21086] to IL-1 beta - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free
    See all IL-1 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR21086] to IL-1 beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LPS treated THP-1 whole cell lysate.

  • General notes

    Ab229696 is the carrier-free version of ab216995. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab229696 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)
    Western blot - Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)
    All lanes : Anti-IL-1 beta antibody [EPR21086] (ab216995) at 1/1000 dilution

    Lane 1 : Wild-type THP-1 untreated cell lysate
    Lane 2 : Wild-type THP-1 LPS-treated (3 h, 100 ng/ml) cell lysate
    Lane 3 : IL1B knockout THP-1 untreated cell lysate
    Lane 4 : IL1B knockout THP-1 LPS-treated (3 h, 100 ng/ml) cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 27-32 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab216995).

    Lanes 1 - 4: Merged signal (red and green). Green - ab216995 observed at 27-32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab216995 was shown to react with IL-1 beta in wild-type THP-1 cells in Western blot with loss of signal observed in IL1B knockout sample. Wild-type THP-1 and IL1B knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab216995 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunoprecipitation - Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)
    Immunoprecipitation - Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)

    IL-1 beta was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours, with ab216995 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216995 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: THP-1 (human monocytic leukemia monocyte) treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours whole cell lysate 10 µg (Input).
    Lane 2: ab216995 IP in THP-1 treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216995 in THP-1 treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216995).

  • Western blot - Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)
    Western blot - Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)
    All lanes : Anti-IL-1 beta antibody [EPR21086] (ab216995) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 2 : THP-1 treated with 100 ng/ml Lipopolysaccharide (LPS) for 3 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    The expression profile is consistent with the literature (PMID 15192144 and 10845914).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216995).

  • Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)
    Anti-IL-1 beta antibody [EPR21086] - BSA and Azide free (ab229696)

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