Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [J10.4] to Junctional Adhesion Molecule 1/JAM-A - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies

  • Description

    Mouse monoclonal [J10.4] to Junctional Adhesion Molecule 1/JAM-A - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human Junctional Adhesion Molecule 1/JAM-A.
    Database link: Q9Y624

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Caco-2, HT-29, A431 and HUVEC whole cell lysate. ICC/IF: HT-29 cells. Flow Cyt: HT-29 and Caco-2 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab267408 is the carrier-free version of ab267407. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab267408 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)
    Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)
    All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407) at 1.035 µg/ml

    Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 4 : HUVEC (human umbilical vein endothelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10698320).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267407).

  • Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)
    Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 cells labelling JAM-A with ab267407 at 20.7µg/ml, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HT-29 cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab267407 at 20.7µg/ml followed by ab150080 at a 1/500 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267407).

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)

    Flow cytometric analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling JAM-A with ab267407 at 1.035µg/ml (Red) compared with a Mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267407).

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)

    Flow cytometric analysis of Caco-2 (Human colorectal adenocarcinoma epithelial cell) cells labelling JAM-A with ab267407 at 1.035µg/ml  (Red) compared with a Mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267407).

  • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] - BSA and Azide free (ab267408)

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