Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-336-1B2] to CD3 zeta - BSA free
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-CD3 zeta antibody [BL-336-1B2] - BSA free
See all CD3 zeta primary antibodies
Description

Rabbit monoclonal [BL-336-1B2] to CD3 zeta - BSA free
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

IP
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CD3 zeta aa 150-164 (C terminal). The exact sequence is proprietary. NP_932170.1 and Gene ID 919.
Database link: P20963
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272071 is the carrier-free version of ab243874. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Application	Species
IHC-P	Human
IP	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-336-1B2
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

CD3 zeta was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243874 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243874 at 1/1000 dilution.

Lane 1: ab243874 IP in Jurkat whole cell lysate.

Lane 2: Contol IgG in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243874).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

Formalin-fixed, paraffin-embedded human metastatic lymph node tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243874).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243874).
Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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