Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP265(2)Y] to CD3 zeta (phospho Y142) - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free
    See all CD3 zeta primary antibodies

  • Description

    Rabbit monoclonal [EP265(2)Y] to CD3 zeta (phospho Y142) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects CD3 zeta phosphorylated on tyrosine 142.

  • Tested applications

    Suitable for: ICC, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat whole cell lysate (ab7899)

  • General notes

    ab245765 is the carrier-free version of ab68235 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab245765 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP265(2)Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
    Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
    All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) treated with 50mM pervanadate for 5 minutes whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68235).

  • Immunocytochemistry - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
    Immunocytochemistry - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) treated with 1mM pervanadate for 30 minutes cells labeling CD3 zeta with purified ab68235 at 1/500 dilution (0.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68235).

  • Flow Cytometry - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
    Flow Cytometry - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) treated with 50nM pervanadate for 5 minutes cells labeling CD3 zeta with purified ab68235 at 1/120 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Untreated Jurkat cells (Green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68235).

  • Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
    Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)

    ab68235 (purified) at 1/20 dilution (0.5ug) immunoprecipitating CD3 zeta in Jurkat treated with 50nM pervanadate for 5 minutes whole cell lysate whole cell lysate. Jurkat (Human T cell leukemia T lymphocyte) treated with 50nM pervanadate for 5 minutes whole cell lysate 10ug
    Lane 2 (+): ab68235 & Jurkat treated with 50nM pervanadate for 5 minutes whole cell lysate whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68235 in Jurkat treated with 50nM pervanadate for 5 minutes whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/2000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68235).

  • Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
    Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)

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