Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI)
See all TFPI kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 7.4% -
Sample type
Cell culture supernatant, Milk, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.12 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Recovery
96.5 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.
A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.
Get results in 90 minutes with Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Tissue Factor Pathway Inhibitor Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Tissue Factor Pathway Inhibitor Microplate (12 x 8 well strips) 1 x 96 units Tissue Factor Pathway Inhibitor Standard 2 vials -
Research areas
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anti convertin
- EPI
- Extrinsic pathway inhibitor
see all -
Database links
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
Images
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Sandwich ELISA - ab108904 Tissue Factor Pathway Inhibitor (TFPI) Human ELISA Kit
TFPI measured in various samples showing quantity (ng) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108904.