Antibodies, Proteins, Kits and Reagents for Life Science

Anti-VISTA antibody [BLR035F] - BSA free (ab272080)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR035F] to VISTA - BSA free
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-VISTA antibody [BLR035F] - BSA free
See all VISTA primary antibodies
Description

Rabbit monoclonal [BLR035F] to VISTA - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human VISTA aa 250-311 (C terminal). The exact sequence is proprietary. NP_071436.1 and Gene ID 64115.
Database link: Q9H7M9
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272080is the carrier-free version of ab243891. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR035F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)

VISTA was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243891 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243891 at 1/1000 dilution.

Lane 1: ab243891 IP in Jurkat whole cell lysate.

Lane 2: Contol IgG in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for VISTA using ab243891 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).
Immunocytochemistry - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)

Formalin-fixed, paraffin-embedded NCI H226 cells labeling VISTA using ab243891 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).
Anti-VISTA antibody [BLR035F] - BSA free (ab272080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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