Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
Key features and details
- Rabbit polyclonal to Histone H4 (acetyl K91) - ChIP Grade
- Suitable for: IHC-P, WB, ChIP
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (acetyl K91) antibody - ChIP Grade
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (acetyl K91) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ChIPmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Rat, Chicken, Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Duck
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Immunogen
Images
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ChIP - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4627 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)IHC image of ab4627 staining Histone H2A.X in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4627, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)Lanes 1 & 3 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/500 dilution
Lanes 2 & 4 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/1000 dilution
Lanes 1-2 : Calf thymus histone prep.
Lanes 3-4 : Calf thymus histone prep. with Human Histone H4 (acetyl K91) peptide (ab10112) at 2 µg
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 12 kDaRabbit polyclonal to Histone H4 acetyl K91 (ab4627). All lanes contain 1ug of calf thymus Histone prep.
Lane 1: ab4627 at 1/500
Lane 2: ab4627 at 1/1000
Lane 3: ab4627 at 1/500 + 2ug acetyl K91 blocking peptide (ab10112)
Lane 4: ab4627 at 1/1000 + 2ug acetyl K91 blocking peptide (ab10112)Secondary antibody: Goat anti-rabbit (HRP) - ab6721 at 1/2000
Predicted Molecular weight11KDa
