Anti-Histone H3 (tri methyl K4) antibody (ab272143)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K4)
- Suitable for: WB, ICC/IF, Dot blot, ChIP
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K4) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K4) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Dot blot, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K4).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo nuclear extract. HeLa and NIH/3T3 histone preperations. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.0004% Potassium phosphate, 0.0008% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 antibody (ab272143)Anti-Histone H3 (tri methyl K4) antibody (ab272143) at 1/500 dilution + Caenorhabditis elegans embryo nuclear extract at 1/30 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody (ab272143)Immunofluorescence analysis of ab272143. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were treated with primary antibody ab272143 at a 1:50 dilution for 1 hour at room temperature. FITC secondary antibody at 1:10,000 for 45 minutes at room temperature. Histone H3 [Trimethyl Lys4] localization is nuclear and chromosomal. Histone H3 K4me3 staining was expressed green, and the nuclei are counterstained blue with DAPI. Fixation was 0.5% PFA abd antigen retrieval was not required.
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Western blot - Anti-Histone H3 antibody (ab272143)All lanes : Anti-Histone H3 (tri methyl K4) antibody (ab272143) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparations
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) histone preparations
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
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ChIP - Anti-Histone H3 antibody (ab272143)Chromatin Immunoprecipitaion of ab272143. Chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was used with 2 µg of ab272143 and 20µl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Dot Blot - Anti-Histone H3 antibody (ab272143)Dot Blot of ab272143.Lane 1: K4.Lane 2: K4-KMe1.Lane 3: K4-KMe2.Lane 4: K4-KMe3.Lane 5: K23-KMe3.Load: 1, 3, and 10 µg of peptide. Primary antibody ab272143 at 1:1000 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1:10,000 for 45 minutes at room temperature.Block: 5% BLOTTO overnight at 4°C.
