Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K4, acetyl K9)
- Suitable for: WB, Dot blot, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K4, acetyl K9) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K4, acetyl K9) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K4, acetyl K9).
Database link: Q71DI3 -
Positive control
- WB: NIH/3T3 cell lysates ICC/IF: HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164)Lane 2 : Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164) at 1 µg/ml
Lane 1 : Histone H3 nuclear marker
Lane 2 : NIH/3T3 cell lysate at 35 µg
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164)Immunofluorescent analysis of HeLa cells labeling Histone H3 (tri methyl K4, acetyl K9) using using ab272164 at 1/10 dilution (Green). The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red. -
Dot Blot - Anti-Histone H3 (tri methyl K4, acetyl K9) antibody (ab272164)Dot Blot analysis of H3 (tri methyl K4, acetyl K9) using ab272164 at 1/1000 dilution.
Lane 1: K4/K9 unmodified.
Lane 2: K4-Me3/K9-Ac.
Lane 3: K4-Me1.
Lane 4: K4-Me2.
Lane 5: K4-Me3.
Lane 6: K9-Ac.
Load: 1, 10, and 100 picomoles of peptide.