Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K4, phospho T6)
- Suitable for: Dot blot, ICC/IF, ChIP, WB
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K4, phospho T6) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K4, phospho T6) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ICC/IF, ChIP, WBmore details -
Species reactivity
Reacts with: Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K4, phospho T6).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo lysate; HeLa histone preparations. ICC/IF: HeLa cells. ChIP: Chromatin from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Do Not Freeze. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144)Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144) at 1/500 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144)Immunofluoescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K4, phospho T6) with ab272144 at a 1/50 dilution for 1 hour at room temperature in ICC/IF (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.
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Western blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144)Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144) at 1/500 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparations at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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ChIP - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144)ChIP of Histone H3 (tri methyl K4, phospho T6). Chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was used with 2 µg of ab272144 to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
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Dot Blot - Anti-Histone H3 (tri methyl K4, phospho T6) antibody (ab272144)Dot Blot analysis of Histone H3 (tri methyl K4, phospho T6) labeled with ab272144 at 1/1000 dilution.
Lane 1: Unmodified.
Lane 2: pT6.
Lane 3: K4Me1pT6.
Lane 4: K4Me2pT6.
Lane 5: K4Me3pT6.
Load: 3, 1, and 0.3 picomoles of peptide.
Block: 5% BLOTTO overnight at 4°C.