Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H4 (acetyl K8) antibody (ab227855)

Key features and details

Rabbit polyclonal to Histone H4 (acetyl K8)
Suitable for: WB, IP, IHC-P, ChIP, Dot blot, ICC/IF
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-Histone H4 (acetyl K8) antibody
See all Histone H4 primary antibodies
Description

Rabbit polyclonal to Histone H4 (acetyl K8)
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Histone H4 (N terminal) (acetyl K8). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P62805
Positive control
- ICC/IF: HeLa cells. IHC-P: Rat hind brain tissue. Mouse kidney and duodenum tissue. WB: HeLa treated with DMSO or Trichostatin A, whole cell lysates. IP: HeLa, treated with TSA, whole cell lysate. ChIP: HeLa chromatin extract treated with Trichostatin A.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-Histone H4 (acetyl K8) antibody (ab227855)

All lanes : Anti-Histone H4 (acetyl K8) antibody (ab227855) at 1/5000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with 0.012% DMSO for 18 hours
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with 0.4 µM Trichostatin A for 18 hours

Lysates/proteins at 30 µg per lane.

Predicted band size: 11 kDa

15% SDS-PAGE gel.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K8) antibody (ab227855)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (acetyl K8) (green) using ab227855 at 1/500 dilution in ICC/IF.

Red: alpha Tubulin stained with an alpha Tubulin antibody at 1/500 dilution.
Blue: Hoechst 33342 staining.
ChIP - Anti-Histone H4 (acetyl K8) antibody (ab227855)

Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract treated with Trichostatin A (0.4 μM for 18 hours) and 5 μg of either control rabbit IgG or ab227855. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Immunoprecipitation - Anti-Histone H4 (acetyl K8) antibody (ab227855)

Histone H4 (acetyl K8) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 2.5 µg ab227855. Western blot was performed from the immunoprecipitate using ab227855. Anti-rabbit IgG (HRP) was used as a secondary reagent.

Lane 1: 30 μg whole cell lysate/extract of Histone H4 (acetyl K8) protein expressing HeLa cells (0.4 µM TSA treatment for 18 hours).

Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.

Lane 3: Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of ab227855.

12% SDS-PAGE gel.

The immunoprecipitated Histone H4 (acetyl K9) protein was detected by WB using ab227855 at a 1/5000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K8) antibody (ab227855)

Paraffin-embedded mouse duodenum tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K8) antibody (ab227855)

Paraffin-embedded mouse kidney tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K8) antibody (ab227855)

Paraffin-embedded rat hind brain tissue stained for Histone H4 (acetyl K8) using ab227855 at 1/500 dilution in immunohistochemical analysis.
Dot Blot - Anti-Histone H4 (acetyl K8) antibody (ab227855)

Dot Blot analysis of ab227855 with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227855 at 1/5000 dilution.
A: Peptide samples of H4K8 un
B: Peptide samples of H4K8ac
C: Peptide samples of H4K16un
D: Peptide samples of H4K16ac

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