Anti-Histone H3 (tri methyl K27) antibody (ab272165)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K27)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K27) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K27) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K27).
Database link: Q71DI3 -
Positive control
- WB: HeLa, NIH/3T3, PC12 and A431 cell lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (methyl K27) antibody (ab272165)All lanes : Anti-Histone H3 (tri methyl K27) antibody (ab272165) at 1 µg/ml
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 5 : A431 (Human epidermoid carcinoma cell line) cell lysate
Predicted band size: 15 kDa
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Immunofluorescence - Anti-Histone H3 (tri methyl K27) antibody (ab272165)Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (methyl K27) using ab272165 at a 1/50 dilution for 1 hour at room temperature (Green). The secondary antibody FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.
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Dot Blot - Anti-Histone H3 (methyl K27) antibody (ab272165)Dot Blot analysis of Histone H3 (methyl K27) using ab272165 at 0.1 μg/ml.Lane 1: Non Modified Peptide.Lane 2: Histone H3 K27-Me1 Peptide.Lane 3: Histone H3 K27-Me2 Peptide.Lane 4: Histone H3 K27-Me3 Peptide.Lane 5: Histone H3 K27-Ac Peptide.Blocking: 5% Blotto at room temperature 30 minutes. Exposure time: 13 seconds.