Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11522(B)] to Ndufs1
Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
Reacts with: Human

Product name

Anti-Ndufs1 antibody [EPR11522(B)]
See all Ndufs1 primary antibodies
Description

Rabbit monoclonal [EPR11522(B)] to Ndufs1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide, corresponding to residues in Human Ndufs1 (UniProt: P28331).
Positive control
- Raji, HepG2, MOLT4 and Jurkat cell lysates; Human kidney and liver tissues; HepG2 cells; Permeabilized Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11522(B)
Isotype

IgG
Research areas

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

All lanes : Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution

Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human normal prostate.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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