Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 102 522 ₸

Order now and get it on Wednesday March 10, 2021

Anti-EBI3 antibody [EPR23372-282] (ab272709)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23372-282] to EBI3
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-EBI3 antibody [EPR23372-282]
See all EBI3 primary antibodies
Description

Rabbit monoclonal [EPR23372-282] to EBI3
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human placenta lysate. His-tagged recombinant human EBI3 protein. IHC-P: Human placenta, tonsil and Human colon carcinoma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23372-282
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] (ab272709)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EBI3 with ab272709 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human placenta (PMID: 21849417). The section was incubated with ab272709 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] (ab272709)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling EBI3 with ab272709 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon carcinoma (PMID: 24040445). The section was incubated with ab272709 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-EBI3 antibody [EPR23372-282] (ab272709)

All lanes : Anti-EBI3 antibody [EPR23372-282] (ab272709) at 1/1000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : Human heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 29 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID: 8551575).

Negative control: Human heart (PMID:8551575).

Exposure time: 3 minutes
Western blot - Anti-EBI3 antibody [EPR23372-282] (ab272709)

Anti-EBI3 antibody [EPR23372-282] (ab272709) at 1/1000 dilution + His-tagged recombinant human EBI3 protein at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.

Exposure time: 7 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [EPR23372-282] (ab272709)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EBI3 with ab272709 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered cells in human tonsil (PMID: 16639698). The section was incubated with ab272709 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-EBI3 antibody [EPR23372-282] (ab272709)

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