Anti-CSN1 antibody [EP15642-22] (ab194359)
![Anti-CSN1 antibody [EP15642-22] (ab194359)](https://www.abcam.com/ps/products/194/ab194359/Images/ab194359-232208-anti-csn1-antibody-ep15642-22-western-blot.jpg "Anti-CSN1 antibody [EP15642-22] (ab194359)")
Key features and details
- Rabbit monoclonal [EP15642-22] to CSN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CSN1 antibody [EP15642-22]
See all CSN1 primary antibodies -
Description
Rabbit monoclonal [EP15642-22] to CSN1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, K562, MCF7 and 293T cell lysates; Mouse brain, kidney, heart and spleen tissue lysates; Rat brain, kidney, heart and spleen tissue lysates; Human bladder transitional cell carcinoma and mouse kidney tissues; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP15642-22 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)All lanes : Anti-CSN1 antibody [EP15642-22] (ab194359) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 56 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CSN1 antibody [EP15642-22] (ab194359)](https://www.abcam.com/ps/products/194/ab194359/Images/ab194359-253291-anti-csn1-antibody-ep15642-22-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CSN1 antibody [EP15642-22] (ab194359)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CSN1 with ab194359 at 1/150 dilution (12 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)](https://www.abcam.com/ps/products/194/ab194359/Images/ab194359-232211-anti-csn1-antibody-ep15642-22-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CSN1 with ab194359 at 1/300 (6μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution (ab97051) and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)](https://www.abcam.com/ps/products/194/ab194359/Images/ab194359-232210-anti-csn1-antibody-ep15642-22-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody [EP15642-22] (ab194359)Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CSN1 with ab194359 at 1/300 (6μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution (ab97051) and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)](https://www.abcam.com/ps/products/194/ab194359/Images/ab194359-232209-anti-csn1-antibody-ep15642-22-western-blot.jpg)
Western blot - Anti-CSN1 antibody [EP15642-22] (ab194359)All lanes : Anti-CSN1 antibody [EP15642-22] (ab194359) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 56 kDa
