Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19697] to RIP - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-RIP antibody [EPR19697] - BSA and Azide free
    See all RIP primary antibodies

  • Description

    Rabbit monoclonal [EPR19697] to RIP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NIH/3T3 cells.

  • General notes

    Ab238451 is the carrier-free version of ab202985. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238451 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)
    Immunoprecipitation - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

    ab202985 Immunoprecipitating RIP in mouse embryo whole cell lysate. 2µg of capture antibody per 0.35mg of lysate was used. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at 1/1000 dilution.

    Lane 1: NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2: NIH/3T3 (mouse embryo) whole cell lysate 
    Lane 3: Rabbit monoclonal IgG ab172730 instead of ab202985 in NIH/3T3 (mouse embryo) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202985).

  • Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)
    Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling RIP with ab202985 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The results show cytoplasmic staining on RAW 264.7 cells. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202985 at 1/200 dilution followed by ab150120  at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202985).

  • Flow Cytometry - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)
    Flow Cytometry - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling RIP with ab202985 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202985).

  • Flow Cytometry - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)
    Flow Cytometry - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

    Flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling RIP with ab202985 at 1/500 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202985).

  • Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)
    Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling RIP with ab202985 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The results show cytoplasmic staining on NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202985 at 1/200 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab202985).

  • Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)
    Anti-RIP antibody [EPR19697] - BSA and Azide free (ab238451)

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