Anti-MER + SKY antibody (ab79223)
Key features and details
- Rabbit polyclonal to MER + SKY
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MER + SKY antibody -
Description
Rabbit polyclonal to MER + SKY -
Host species
Rabbit -
Specificity
Detects endogenous levels of total MER + SKY protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic non-phosphopeptide derived from human MER + SKY around the phosphorylation site of tyrosine 749/681 (K-I-YP-S-G).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MER + SKY antibody (ab79223)Left panel: immunohistochemistry analysis of paraffin-embedded Human brain tissue using 1/50 ab79223. Right panel: same, after pre-incubation with the immunizing peptide.
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Western blot - Anti-MER + SKY antibody (ab79223)All lanes : Anti-MER + SKY antibody (ab79223) at 1/500 dilution
Lane 1 : 3T3 cell extract treated
with EGF (200ng/ml, 5mins)
Lane 2 : 3T3 cell extract treated
with EGF (200ng/ml, 5mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 , 97 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 150 kDa. We are unsure as to the identity of these extra bands.