Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4689] to RIP - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-RIP antibody [EPR4689] - BSA and Azide free
    See all RIP primary antibodies

  • Description

    Rabbit monoclonal [EPR4689] to RIP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment aa 300-450 (internal sequence).

  • General notes

    ab234924 is the carrier-free version of ab125072 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab234924 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

    This data was developed using ab125072, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: RIP knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: Raji cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab125072 (unpurified) observed at 78 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab125072 was shown to specifically react with RIP in wild-type HAP1 cells. No band was observed when RIP knockout samples were examined. Wild-type and RIP knockout samples were subjected to SDS-PAGE. ab125072 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Anti-RIP antibody [EPR4689] (ab125072) at 1/5000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    This data was developed using ab125072, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Anti-RIP antibody [EPR4689] (ab125072) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    This data was developed using ab125072, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    All lanes : Anti-RIP antibody [EPR4689] (ab125072) at 1/1000 dilution (unpurified)

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 75 kDa



    This data was developed using ab125072, the same antibody clone in a different buffer formulation.

     

  • Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
    Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

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