Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)
![Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)](https://www.abcam.com/ps/products/184/ab184945/Images/ab184945-217726-ab184945WB.jpg "Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11647(2)(B)] to FH/Fumarase - N-terminal
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal
See all FH/Fumarase primary antibodies -
Description
Rabbit monoclonal [EPR11647(2)(B)] to FH/Fumarase - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa, 293 and A549 cells lysates; HeLa cells.
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General notes
This product was previously labelled as FH
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11647(2)(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)All lanes : Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)](https://www.abcam.com/ps/products/184/ab184945/Images/ab184945-217725-ab184945IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)Immunofluorescence analysis of, -20 °C acetone-fixed, HeLa cells labeling FH/Fumarase with ab184945 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
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![Flow Cytometry - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)](https://www.abcam.com/ps/products/184/ab184945/Images/ab184945-217724-ab184945FACS.jpg)
Flow Cytometry - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)Flow cytometry analysis of 2% paraformaldehyde-fixed HeLa cells labeling FH/Fumarase with ab184945 at a 1/160 dilution (red) or negative control rabbit IgG (green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.
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![Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)](https://www.abcam.com/ps/products/184/ab184945/Images/ab184945-4-benefits-of-recombinant-antibodies.png)
Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945)
