Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21105] to FH/Fumarase - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free
    See all FH/Fumarase primary antibodies

  • Description

    Rabbit monoclonal [EPR21105] to FH/Fumarase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Rat
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney and clear cell renal cancer tissue.

  • General notes

    ab234907 is the carrier-free version of ab233393 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab234907 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as FH

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling FH/Fumarase with ab233393 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular, cytoplasmic and nuclear staining on rat kidney (PMID: 17111171) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FH/Fumarase with ab233393 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Intensive granular and weak nuclear staining in mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Immunoprecipitation - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Immunoprecipitation - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    FH/Fumarase was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab233393 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233393 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HEK-293 whole cell lysate 10 μg (Input).
    Lane 2: ab233393 IP in HEK-293 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233393 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Flow Cytometry - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Flow Cytometry - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling FH/Fumarase with ab233393 at 1/500 dilution (red) compared with an Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling FH/Fumarase with ab233393 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling FH/Fumarase with ab233393 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue (left) and human clear cell renal cancer tissue (right) tissue labeling FH/Fumarase with ab233393 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Intense granular staining on human kidney whereas weaker staining in clear cell renal cancer tissue (PMID: 21695080) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233393).

  • Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)
    Anti-FH/Fumarase antibody [EPR21105] - BSA and Azide free (ab234907)

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