Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21104] to FH/Fumarase
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-FH/Fumarase antibody [EPR21104]
See all FH/Fumarase primary antibodies
Description

Rabbit monoclonal [EPR21104] to FH/Fumarase
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain and fetal kidney lysates; RAW 264.7, HepG2, HeLa, C6, NIH/3T3, Jurkat, MCF7 and HEK-293 whole cell lysates. IHC-P: Human clear cell renal cancer tissue; Human, mouse and rat kidney tissues. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
This product was previously labelled as FH

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21104
Isotype

IgG
Research areas

Western blot - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

All lanes : Anti-FH/Fumarase antibody [EPR21104] (ab233394) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 9 : Human brain lysate
Lane 10 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 9-10 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 54 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time : Lanes 1-5: 26 seconds; Lanes 5-6: 10 seconds; Lane 8-10: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Immunohistochemical analysis of paraffin-embedded human kidney tissue (left panel) and human clear cell renal cancer tissue (right panel) labeling FH/Fumarase with ab233394 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Intense granular staining on human kidney whereas weaker staining in clear cell renal cancer (PMID: 21695080). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Immunofluorescent analysis of 100% methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FH/Fumarase with ab233394 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling FH/Fumarase with ab233394 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary
Immunoprecipitation - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

FH/Fumarase was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab233394 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233394 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).

Lane 2: ab233394 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233394 in HeLa whole cell lysate.

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FH/Fumarase with ab233394 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular staining on mouse kidney is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling FH/Fumarase with ab233394 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular staining on rat kidney is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR21104] (ab233394)

Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling FH/Fumarase with ab233394 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-FH/Fumarase antibody [EPR21104] (ab233394)

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