Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pan PDK antibody [3H5BE12] (ab115321)

Key features and details

  • Mouse monoclonal [3H5BE12] to pan PDK
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-pan PDK antibody [3H5BE12]

  • Description

    Mouse monoclonal [3H5BE12] to pan PDK

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • General notes

    This antibody clone is manufactured by Abcam.

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)
    Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)
    All lanes : Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/ml

    Lane 1 : human heart mitochondria at 20 µg
    Lane 2 : human liver mitochondria at 10 µg
    Lane 3 : rat liver mitochondria at 10 µg
    Lane 4 : mouse liver mitochondria at 10 µg
    Lane 5 : PDK1 recombinant protein ab110359
    at 0.1 µg
    Lane 6 : PDK2 recombinant protein ab110354 at 0.1 µg
    Lane 7 : PDK3 recombinant protein ab110355 at 0.1 µg
    Lane 8 : PDK4 recombinant protein ab110356 at 0.1 µg
    Lane 9 : PDK1 recombinant protein ab86986 at 0.1 µg

    Secondary
    All lanes : GAM IR 800 1:5000

    Performed under reducing conditions.

    Predicted band size: 49 kDa



    ab115321 identifies all four isoforms of PDKs (PDK1, PDK2, PDK3, and PDK4) with differential affinity. PDK3 has the highest affinity, then PDK2 and 4, it has the lowest affinity to PDK1.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (ab115321)
    IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321)
    Western blot - Anti-pan PDK antibody [3H5BE12] (ab115321) This image is courtesy of an anonymous Abreview

    Western blot analysis of human ovarian cancer cell lines whole cell lysate (30µg/lane) labelling pan PDK with ab115321 at 1/1000. A HRP-conjugated goat anti-mouse IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

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