Anti-DHODH antibody (ab246893)
Key features and details
- Rabbit polyclonal to DHODH
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHODH antibody
See all DHODH primary antibodies -
Description
Rabbit polyclonal to DHODH -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Orangutan
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Immunogen
Recombinant fragment corresponding to Human DHODH aa 200-350.
Database link: Q02127 -
Positive control
- IHC-P: Human kidney, liver, endometrium and duodenum tissue. WB: DHODH over expressed HEK-239T lysate. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DHODH antibody (ab246893)
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for DHOHD (green) using ab246893 at 4 μg/ml in ICC/IF.
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Western blot - Anti-DHODH antibody (ab246893)All lanes : Anti-DHODH antibody (ab246893) at 0.4 µg
Lane 1 : Vector only-transfected HEK-293T cell lysate
Lane 2 : DHODH over-expression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 42 kDa
Western blot analysis in control (vector only transfected HEK293T lysate) and DHODH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419975). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHODH antibody (ab246893)Formalin-fixed, paraffin-embedded human endometrium tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHODH antibody (ab246893)Formalin-fixed, paraffin-embedded human kidney tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHODH antibody (ab246893)Formalin-fixed, paraffin-embedded human duodenum tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHODH antibody (ab246893)Formalin-fixed, paraffin-embedded human liver tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.