Rat GSTA1 ELISA Kit (ab254508)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.35 ng/ml
- Range: 0.8 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat GSTA1 ELISA Kit
See all GSTA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 5.4% Inter-assay Sample n Mean SD CV% Supernatant 3 7.7% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
0.35 ng/ml -
Range
0.8 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 102 95% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat GSTA1 ELISA Kit (ab254508) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GSTA1 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GSTA1 with 0.35 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Glutathione S-transferase alpha-1 (GSTA1) is an enzyme in the GST family and is encoded by the GSTA1 gene. GSTA1s main role is the detoxification of electrophilic compounds, including carcinogens, drugs, environmental toxins, and by-products on environmental stress by conjugation with reduced glutathione. The alpha series of enzymes for GSH are activated in the liver. Rat GSTA1 shares a 96% sequence identify with GSTA2, and 94.6% with mouse GSTA1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat GSTA1 Capture Antibody 1 x 600µl 10X Rat GSTA1 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat GSTA1 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Domain
The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Glutathione S alkyltransferase A1
- Glutathione S aryltransferase A1
- Glutathione S transferase 2
see all -
Database links
- Entrez Gene: 24421 Rat
- SwissProt: P00502 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat GSTA1 standard curve in Sample Diluent NS.The GSTA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GSTA1 in rat cell culture supernatant samples.The concentrations of GSTA1 were measured in duplicates, interpolated from the GSTA1 standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 1:13,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GSTA1 concentration was determined to be 271 µg/mL in liver supernatant.
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Sandwich ELISA - Rat GSTA1 ELISA Kit (ab254508)To learn more about the advantages of recombinant antibodies see here.
