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Signal Transduction Metabolism Energy Metabolism

Anti-PKM antibody (ab131021)

Anti-PKM antibody (ab131021)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PKM
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PKM antibody
    See all PKM primary antibodies
  • Description

    Rabbit polyclonal to PKM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PKM aa 1-50.
    Database link: NP_002645.3

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131021 is affinity purified using an epitope specific to PKM2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-PKM antibody (ab131021)
    Western blot - Anti-PKM antibody (ab131021)
    All lanes : Anti-PKM antibody (ab131021) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 58 kDa


    Exposure time: 3 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab131021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab131021)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human osteosarcoma tissue labelling PKM with ab131021 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab131021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab131021)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling PKM with ab131021 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-PKM antibody (ab131021)
    Immunoprecipitation - Anti-PKM antibody (ab131021)

    Detection of PKM2 by Western Blot of Immunprecipitate.

    ab131021 at 0.4µg/ml staining PKM in HeLa whole cell lysate immunoprecipitated using ab131021 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure time of 3 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PKM antibody (ab131021)

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    Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)

    Applications: Flow Cyt, ICC/IF, WB

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    Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)

    Applications: ICC/IF

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    Anti-PKM antibody [EPR10138(B)] (ab150377)

    Applications: Flow Cyt, ICC/IF, WB

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    Anti-PKM antibody [EPR10139] (ab154816)

    Applications: IHC-P, WB

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    Anti-PKM antibody (ab137791)

    Applications: IHC-P, IP, WB

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    PE Anti-PKM antibody [EPR10138(B)] (ab210448)

    Applications: Flow Cyt, ICC/IF

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