Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10138(B)] to PKM - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKM antibody [EPR10138(B)] - BSA and Azide free
    See all PKM primary antibodies

  • Description

    Rabbit monoclonal [EPR10138(B)] to PKM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle, HeLa, Jurkat and A549 cell lysates. Mouse brain, heart, kidney, and spleen tissue lysates. Rat brain, heart, and spleen tissue lysates; ICC/IF: HeLa cells; Flow Cyt: HeLa cells.

  • General notes

    Ab206129 is the carrier-free version of ab150377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab206129 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)
    Western blot - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)
    All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 180 seconds


    This data was developed using ab150377, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)
    Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)

    This ICC data was generated using the same anti-PKM antibody clone, EPR10138(B), in a different buffer formulation (cat# ab150377).

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Flow Cytometry - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)
    Flow Cytometry - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)

    This Flow cytometry data was generated using the same anti-PKM antibody clone, EPR10138(B), in a different buffer formulation (cat# ab150377).

    Flow cytometric analysis of permeabilized HeLa cells labeling PKM with ab150377 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)
    Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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