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Signal Transduction Metabolism Energy Metabolism

Anti-PKM antibody - N-terminal (ab196560)

Anti-PKM antibody - N-terminal (ab196560)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PKM - N-terminal
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PKM antibody - N-terminal
    See all PKM primary antibodies
  • Description

    Rabbit polyclonal to PKM - N-terminal
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total PKM protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PKM2 (N terminal).
    Database link: P14618

  • Positive control

    • Jurkat, HeLa and A549 extracts. Human, mouse and rat lung cancer tissue. HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-PKM antibody - N-terminal (ab196560)
    Western blot - Anti-PKM antibody - N-terminal (ab196560)
    All lanes : Anti-PKM antibody - N-terminal (ab196560) at 1/500 dilution

    Lane 1 : Jurkat extracts
    Lane 2 : HeLa extracts
    Lane 3 : A549 extracts

    Predicted band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody - N-terminal (ab196560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody - N-terminal (ab196560)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling PKM2 with ab196560 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody - N-terminal (ab196560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody - N-terminal (ab196560)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling PKM2 with ab196560 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody - N-terminal (ab196560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody - N-terminal (ab196560)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling PKM2 with ab196560 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody - N-terminal (ab196560)
    Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody - N-terminal (ab196560)

    Immunofluorescence analysis of HeLa cells labeling PKM2 using ab196560, at 1/20 dilution. Lower panel shows DAPI for nuclear staining (Blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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