Anti-PKM antibody - N-terminal (ab196560)
Key features and details
- Rabbit polyclonal to PKM - N-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PKM antibody - N-terminal
See all PKM primary antibodies -
Description
Rabbit polyclonal to PKM - N-terminal -
Host species
Rabbit -
Specificity
This antibody detects endogenous level of total PKM protein. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PKM2 (N terminal).
Database link: P14618 -
Positive control
- Jurkat, HeLa and A549 extracts. Human, mouse and rat lung cancer tissue. HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PKM antibody - N-terminal (ab196560) at 1/500 dilution
Lane 1 : Jurkat extracts
Lane 2 : HeLa extracts
Lane 3 : A549 extracts
Predicted band size: 58 kDa
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Immunohistochemical analysis of paraffin-embedded human lung tissue labeling PKM2 with ab196560 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling PKM2 with ab196560 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling PKM2 with ab196560 at 1/100 dilution.
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Immunofluorescence analysis of HeLa cells labeling PKM2 using ab196560, at 1/20 dilution. Lower panel shows DAPI for nuclear staining (Blue).