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Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14660] to IKK gamma/NEMO
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IKK gamma/NEMO antibody [EPR14660]
    See all IKK gamma/NEMO primary antibodies

  • Description

    Rabbit monoclonal [EPR14660] to IKK gamma/NEMO

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HeLa, Jurkat, K572 and human fetal brain lysates. ICC/IF: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IKK gamma/NEMO knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab188569 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab188569 was shown to react with IKK gamma/NEMO in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IKK gamma/NEMO knockout samples were examined. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. ab188569 and ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Immunocytochemical analysis of Jurkat cells fixed in 4% paraformaldehyde labeling IKK gamma/NEMO with ab188569 at 1/100 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.  Counterstained with DAPI.

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    All lanes : Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : IKBKG knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab188569 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab188569 was shown to react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in knockout cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and IKBKG knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188569 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    All lanes : Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Additional bands at: 41 kDa (possible cleavage fragment)

  • Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)
    Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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