Anti-Synaptotagmin VII antibody (ab106618)
Key features and details
- Rabbit polyclonal to Synaptotagmin VII
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Synaptotagmin VII antibody
See all Synaptotagmin VII primary antibodies -
Description
Rabbit polyclonal to Synaptotagmin VII -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide corresponding to Human Synaptotagmin VII aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab154624) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications RabbitHumanChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent - 3%.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent - 3%.Target
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Function
May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. -
Tissue specificity
Expressed in a variety of adult and fetal tissues. -
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
Domain
The second C2 domain/C2B is responsible for SYNCRIP binding. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. - Information by UniProt
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Database links
- Entrez Gene: 9066 Human
- Entrez Gene: 54525 Mouse
- Entrez Gene: 59267 Rat
- Omim: 604146 Human
- SwissProt: O43581 Human
- SwissProt: Q9R0N7 Mouse
- Unigene: 502730 Human
- Unigene: 684589 Human
see all -
Alternative names
- IPCA 7 antibody
- IPCA-7 antibody
- IPCA7 antibody
see all
Images
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Western blot - Anti-Synaptotagmin VII antibody (ab106618)All lanes : Anti-Synaptotagmin VII antibody (ab106618) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Cortex Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45,63,68 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa, 76 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The bands observed are representative of Synaptotagmin VII splice variants alpha, beta, and delta. While these differ to its predicted molecular weight of 45 kDa, this migration has been observed in the literature (PMID:17709608) This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106618 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab106618 has been referenced in 1 publication.
- Sidoryk-Wegrzynowicz M et al. Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions. Acta Neuropathol Commun 5:89 (2017). WB . PubMed: 29187256
Images
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Western blot - Anti-Synaptotagmin VII antibody (ab106618)All lanes : Anti-Synaptotagmin VII antibody (ab106618) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Cortex Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45,63,68 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa, 76 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The bands observed are representative of Synaptotagmin VII splice variants alpha, beta, and delta. While these differ to its predicted molecular weight of 45 kDa, this migration has been observed in the literature (PMID:17709608) This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106618 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
