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Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins

Anti-Synaptotagmin VII antibody [N275/14] (ab174633)

Price and availability

264 681 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Synaptotagmin VII antibody [N275/14] (ab174633)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [N275/14] to Synaptotagmin VII
  • Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Synaptotagmin VII antibody [N275/14]
    See all Synaptotagmin VII primary antibodies
  • Description

    Mouse monoclonal [N275/14] to Synaptotagmin VII
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Mouse Synaptotagmin VII aa 150-239 (internal sequence).
    Sequence:

    STLTVKVMKAQELPAKDFSGTSDPFVKIYLLPDKKHKLETKVKRKNLNPH WNETFLFEGFPYEKVVQRVLYLQVLDYDRFSRNDPIGEVS


    Database link: Q9R0N7
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    Cytoplasmic C2A domain
  • Positive control

    • WB: Rat brain lysate.
  • General notes

    Abcam is committed to ensuring that our customers have access to all of the information we have on our products and that the product information is accurate. The clone name for this product was previously known as S27514. However, it has come to our attention that the original and primary clone name is N275/14 so we have therefore changed the product name accordingly.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N275/14
  • Isotype

    IgG2b
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin VII antibody [N275/14] (ab174633)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin VII antibody [N275/14] (ab174633)

    Immunocytochemistry/Immunofluorescence analysis using ab174633 at 1:100 in Neuroblastoma cell line (SK-N-BE) Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubaitionfor 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain at 1:1000 for 60 min RT; DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174633 (D) Composite.

  • Western blot - Anti-Synaptotagmin VII antibody [N275/14] (ab174633)
    Western blot - Anti-Synaptotagmin VII antibody [N275/14] (ab174633)
    Lane 1 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/100 dilution
    Lane 2 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/250 dilution
    Lane 3 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/500 dilution
    Lane 4 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/1000 dilution

    All lanes : Rat brain lysates

    Predicted band size: 45 kDa
    Additional bands at: 65 kDa (possible isoform)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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