Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4577] to Hsp105/HSP110
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Hsp105/HSP110 antibody [EPR4577]
    See all Hsp105/HSP110 primary antibodies

  • Description

    Rabbit monoclonal [EPR4577] to Hsp105/HSP110

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, and Caco-2 whole cell lysate. Human lung tissue lysate; IHC-P: Human testis tissue; ICC/IF: HeLa and HepG2 cells; Flow Cyt: HepG2 cells; IP: HeLa whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Hsp105.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4577

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    All lanes : Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : Human lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 97 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hsp105/HSP110 with Purified ab108625 at 1:50 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Flow Cytometry - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells, labeling Hsp105/HSP110 with Purified ab108625 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor®  488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

    Purified ab108625 at 1:20 dilution (0.6μg) immunoprecipitating Hsp105/HSP110 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab108625 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108625 in HeLa whole cell lysate.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 105 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

    ab108625, unpurified, at 1/100 dilution staining Hsp105/HSP110 in Human testis by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

    ab108625, unpurifed, at 1/100 dilution staining Hsp105/HSP110 in HeLa cells by Immunofluorescence.

  • Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)
    Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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