Anti-Hsp105/HSP110 antibody (ab223620)
Key features and details
- Rabbit polyclonal to Hsp105/HSP110
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp105/HSP110 antibody
See all Hsp105/HSP110 primary antibodies -
Description
Rabbit polyclonal to Hsp105/HSP110 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Human
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Immunogen
Synthetic peptide corresponding to Hamster Hsp105/HSP110. Sequence identical to human and mouse.
Database link: Q60446 -
Positive control
- IHC-P: Mouse backskin.
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General notes
This product was previously labelled as Hsp105
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody (ab223620)
Bouin’s solution-fixed mouse backskin stained for Hsp105/HSP110 (green) using ab223620 at 1/100 dilution in immunohistochemical analysis. Secondary Antibody: Goat Anti-Rabbit FITC at 1/50 for 1 hour at RT.
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Western blot - Anti-Hsp105/HSP110 antibody (ab223620)Anti-Hsp105/HSP110 antibody (ab223620) at 1/1000 dilution + Human cell line lysates at 15 µg
Secondary
Donkey Anti-Rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 92,97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking buffer: 1.5% BSA.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
Mix of human cell line lysates: epidermoid carcinoma (A431), adenocarcinomic alveolar basal epithelial cells (A549), colon cancer (HCT116), cervical cancer (HeLa), embryonic kidney (HEK293), liver cancer (HepG2), promyelocytic leukemia (HL-60), umbilical vein endothelial cells (HUVEC), T lymphocytes (Jurkat), breast cancer (MCF7), prostate cancer (PC3), brain glioblastom (T98G).
