Anti-MMP2 antibody [6E3F8] (ab86607)
![Anti-MMP2 antibody [6E3F8] (ab86607)](https://www.abcam.com/ps/products/86/ab86607/Images/ab86607-101698-anti-mmp2-antibody-6e3f8-western-blot.jpg "Anti-MMP2 antibody [6E3F8] (ab86607)")
Key features and details
- Mouse monoclonal [6E3F8] to MMP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-MMP2 antibody [6E3F8]
See all MMP2 primary antibodies -
Description
Mouse monoclonal [6E3F8] to MMP2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human MMP2 aa 450 to the C-terminus.
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Positive control
- This antibody gave a positive signal in the following lysates: Human Ovary Tissue; Human Liver Tissue; Mouse Liver Tissue; Rat Spleen Tissue; A375 Whole Cell; NIH3T3 Whole Cell; PC12 Whole Cell.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
6E3F8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)All lanes : Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Spleen (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)](https://www.abcam.com/ps/products/86/ab86607/Images/ab86607-101696-anti-mmp2-antibody-6e3f8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)IHC image of ab86607 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86607, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)](https://www.abcam.com/ps/products/86/ab86607/Images/ab86607-295183-anti-mmp2-antibody-6e3f8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607) Image from Sun H et al., J Cell Mol Med. 2015;19(4):836-49. Fig 6.; doi: 10.1111/jcmm.12521. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Immunohistochemical (parraffin-embedded sections) staining for MMP-2 in atheroslerotic lesions of aortic root in from right to left Ad-MIFi, Ad-EGFP, NS control and non-DM mouse control groups using ab86607 at a dilution of 1/200.
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![Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)](https://www.abcam.com/ps/products/86/ab86607/Images/ab86607-233353-anti-mmp2-antibody-6e3f8-western-blot.jpg)
Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)All lanes : Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab86607 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)](https://www.abcam.com/ps/products/86/ab86607/Images/ab86607-308615-anti-mmp2-antibody-6e3f8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)Immunohistochemistry of human pancreas carcinoma staining MMP2 with ab86607 at 1μg/ml.
