Anti-SIN1 (phospho T86) antibody (ab277787)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal to SIN1 (phospho T86)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-SIN1 (phospho T86) antibody
See all SIN1 primary antibodies -
Description
Rabbit recombinant multiclonal to SIN1 (phospho T86) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SIN1 aa 50-150 (phospho T86).
Database link: Q9BPZ7 -
Positive control
- WB: A549, A549 treated with Insulin (Serum starved for 24hrs, 100nM-10min), PC-3, PC-3 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extract.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIN1 antibody (ab277787)All lanes : Anti-SIN1 (phospho T86) antibody (ab277787) at 2 µg/ml
Lane 1 : A549 whole cell extracts
Lane 2 : A549 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extracts
Lane 3 : PC-3 whole cell extracts
Lane 4 : PC-3 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extracts
Lane 5 : A549 whole cell extracts with Phosphopeptide at 10 µg/ml
Lane 6 : A549 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extracts with Phosphopeptide at 10 µg/ml
Lane 7 : PC-3 whole cell extract with Phosphopeptide at 10 µg/ml
Lane 8 : PC-3 treated with Insulin (Serum starved for 24hrs, 100nM-10min) whole cell extract with Phosphopeptide at 10 µg/ml
Lysates/proteins at 30 µg per lane.
Secondary
Lanes 1-4 : HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal, 0.4 µg/mL at 1/2500 dilution
Predicted band size: 59 kDa
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Anti-SIN1 antibody (ab277787)